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corning® cell culture ultra-low attachment cellstack® chamber  (Corning Life Sciences)

 
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    Corning Life Sciences corning® cell culture ultra-low attachment cellstack® chamber
    Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® <t>CellSTACK</t> ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.
    Corning® Cell Culture Ultra Low Attachment Cellstack® Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning%C2%AE+cell+culture+ultra-low+attachment+cellstack%C2%AE+chamber/pmc03283529-148-42-42?v=Corning+Life+Sciences
    Average 90 stars, based on 1 article reviews
    corning® cell culture ultra-low attachment cellstack® chamber - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate"

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    Journal: Journal of Translational Medicine

    doi: 10.1186/1479-5876-9-198

    Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.
    Figure Legend Snippet: Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.

    Techniques Used: Comparison, Cell Culture



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    corning® cell culture ultra-low attachment cellstack® chamber  (Corning Life Sciences)
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    Corning Life Sciences corning® cell culture ultra-low attachment cellstack® chamber
    Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® <t>CellSTACK</t> ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.
    Corning® Cell Culture Ultra Low Attachment Cellstack® Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning%C2%AE+cell+culture+ultra-low+attachment+cellstack%C2%AE+chamber/pmc03283529-148-42-42?v=Corning+Life+Sciences
    Average 90 stars, based on 1 article reviews
    corning® cell culture ultra-low attachment cellstack® chamber - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.

    Article Snippet: As each brand of cell factory might have different surface coating properties that could affect DC differentiation and maturation, three commercially available cell factories were evaluated - 1) NunclonTMΔ Surface 1-tray Cell Factories; 2) Corning ® CellSTACK ® culture chambers; and 3) Corning ® cell culture ultra-low attachment CellSTACK ® chamber.

    Techniques: Comparison, Cell Culture